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Anti-GSK3B PHOSPHO Antibody EPR2286Y
Also for GSK3B (NM_002093)
|A phospho-specific peptide corresponding to residues surrounding Serine 9 of human GSK3 beta was used as an immunogen. The antibody only detects GSK3 beta phosphorylated on Serine 9.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens glycogen synthase kinase 3 beta (GSK3B), transcript variant 1|
|GSK3beta isoform; glycogen synthase kinase-3 beta; glycogen synthase kinase 3 beta|
|Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]. |
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Western blot - GSK3 beta (phospho S9) antibody [EPR2286Y]; All lanes : Anti-GSK3 beta (phospho S9) antibody [EPR2286Y] at 1/20000 dilution.Lane 1 : 293T cell lysates, untreated.Lane 2 : 293T cell lysates, treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 47 kDa.Observed band size : 47 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 beta (phospho S9) antibody [EPR2286Y]; ab75814 at 1/100 dilution staining GSK3 beta in human ovarian carcinoma by Immunohistochemistry, Paraffin-embedded tissue.