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Anti-PDK1 PHOSPHO Antibody EP569Y
Also for PDK1 (NM_002610)
|A synthetic phospho-peptide corresponding to residues surrounding Tyr373 and Tyr376 of human PDK1 was used as immunogen. The antibody only detects PDK1 phosphorylated on Tyrosine 373 and 376.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF or IP.
|Homo sapiens pyruvate dehydrogenase kinase, isozyme 1 (PDK1), transcript variant 2|
|mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1|
|Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. [provided by RefSeq]. |
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Western blot - PDPK1 antibody [EP569Y]; Anti-PDPK1 antibody [EP569Y] at 1/1000 dilution + 293 cell lysate.Predicted band size : 63 kDa.Observed band size : 63 kDa.
Immunohistochemistry (Paraffin-embedded sections) - PDPK1 antibody [EP569Y]; Ab52893 (1:100) staining human PDPK1 in human prostate carcinoma tissue by immunohistochemistry using paraffin embedded tissue.