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Anti-EGFR PHOSPHO Antibody EP39IHCY
Also for EGFR (NM_005228)
|A synthetic peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. This antibody detects both phosphorylated and non-phosphorylated EGFR protein.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:5000; FC: 1:55
|Does not react with Rat. Is unsuitable for IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - EGFR antibody [EP39IHCY]; Anti-EGFR antibody [EP39IHCY] at 1/5000 dilution + HeLa cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 134 kDa.Observed band size : 170,180 kDa .
Immunocytochemistry/ Immunofluorescence - EGFR antibody [EP39IHCY]; ab76153 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.