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Home Antibody All anti-HIST1H3F antibodies

Anti-HIST1H3F PHOSPHO Antibody EP1702Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303821
  • Rabbit monoclonal antibody against Histone H3 (pT3) Phospho (EP1702Y ) (phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for HIST1H3F (NM_021018)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3 was used as an immunogen. This antibody detects Histone H3 phosphorylated at Theronine 3.
Clone NameEP1702Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:100; WB: 1:500000 - 1:1e+006; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens histone cluster 1, H3f (HIST1H3F)
Alternative NameH3/i; H3FI
Database LinkNP_066298
Entrez Gene 8968 Human
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3 (phospho T3) antibody; All lanes : Anti-Histone H3 (phospho T3) antibody at 1/500000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : HeLa cell lysates treated with FBS + Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 14 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (phospho T3) antibody; TA303821 at 1/100 dilution staining Histone H3 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
FC Image
Flow Cytometry-Anti-Histone H3 (phospho T3) antibody(TA303821); Overlay histogram showing HeLa cells stained with TA303821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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