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Home Antibody All anti-HIST1H3F antibodies

Anti-HIST1H3F Antibody EP964Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303819
  • Rabbit monoclonal antibody against Histone H3 Acetyl (K14) (EP964Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412143) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(2)
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Also for HIST1H3F (NM_021018)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3TB acetylated on K14.
Clone NameEP964Y IsotypeIgG
Species ReactivityRat, Human, Saccharomyces cerevisiae, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2000; IHC: 1:100 - 250; ICC: 1:250 - 500; IP: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens histone cluster 1, H3f (HIST1H3F)
Alternative NameH3/i; H3FI
Database LinkNP_066298
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3 (acetyl K14) antibody [EP964Y]; All lanes : Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade at 1/2000 dilution.Lane 1 : C6 cell lysates untreated.Lane 2 : C6 cell lysates treated with TSA.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (acetyl K14) antibody [EP964Y]; TA303819 at 1/100-1/250 dilution staining Histone H3 (acetyl K14) in human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K14) antibody [EP964Y]; TA303819 at 1/250-1/500 dilution staining Histone H3 (acetyl K14) in HeLa cells by Immunofluorescence.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade; ICC/IF image of TA303819 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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