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Home Antibody All anti-HIST1H3F antibodies

Anti-HIST1H3F Antibody EP964Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303819
  • Rabbit monoclonal antibody against Histone H3 Acetyl (K14) (EP964Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC412143) , 20ug
100ul $325 In Stock
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WB(1)
IHC(1)
IF(2)
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Also for HIST1H3F (NM_021018)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3TB acetylated on K14.
Clone NameEP964Y IsotypeIgG
Species ReactivityHuman, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2000; IHC: 1:100 - 250; ICC: 1:250 - 500; IP: 1:20
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens histone cluster 1, H3f (HIST1H3F)
Alternative NameH3/i; H3FI
Database LinkNP_066298
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Related Pathway

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WB Image
Western blot - Histone H3 (acetyl K14) antibody [EP964Y]; All lanes : Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade at 1/2000 dilution.Lane 1 : C6 cell lysates untreated.Lane 2 : C6 cell lysates treated with TSA.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (acetyl K14) antibody [EP964Y]; TA303819 at 1/100-1/250 dilution staining Histone H3 (acetyl K14) in human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K14) antibody [EP964Y]; TA303819 at 1/250-1/500 dilution staining Histone H3 (acetyl K14) in HeLa cells by Immunofluorescence.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade; ICC/IF image of TA303819 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

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