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Anti-HIST1H3F Antibody EP964Y
Also for HIST1H3F (NM_021018)
|A synthetic acetyl-specific peptide corresponding to residues around Lysine 14 was used as an immunogen. This antibody recognizes Histone H3TB acetylated on K14.|
|Rat, Human, Saccharomyces cerevisiae, Caenorhabditis elegans, Fruit fly (Drosophila melanogaster), Schizosaccharomyces pombe (Does not react with: Mouse)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:2000; IHC: 1:100 - 250; ICC: 1:250 - 500; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse
|Homo sapiens histone cluster 1, H3f (HIST1H3F)|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
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Western blot - Histone H3 (acetyl K14) antibody [EP964Y]; All lanes : Anti-Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade at 1/2000 dilution.Lane 1 : C6 cell lysates untreated.Lane 2 : C6 cell lysates treated with TSA.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 11 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (acetyl K14) antibody [EP964Y]; TA303819 at 1/100-1/250 dilution staining Histone H3 (acetyl K14) in human adenocarcinoma of uterus by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K14) antibody [EP964Y]; TA303819 at 1/250-1/500 dilution staining Histone H3 (acetyl K14) in HeLa cells by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K14) antibody [EP964Y] - ChIP Grade; ICC/IF image of TA303819 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.