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Anti-HIST2H4B Antibody EP1000Y
Also for HIST2H4B (NM_001034077)
|A synthetic peptide corresponding to residues near the N-terminus of human Histone H4 was used as an immunogen|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ChIP: Use at an assay dependent dilution; WB: 1:10000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens histone cluster 2, H4b (HIST2H4B)|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq]. |
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Western blot - Histone H4 (acetyl K5) antibody [EP1000Y]; All lanes : Anti-Histone H4 (acetyl K5) [EP1000Y] antibody - ChIP Grade at 1/1000000 dilution.Lane 1 : Untreated HeLa cells.Lane 2 : TSA treated HeLa calls.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP labelled (1:2000).Predicted band size : 11 kDa.Observed band size : 11 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Histone H4 (acetyl K5) antibody [EP1000Y]; Ab51997 (1:500) staining human histone H4 on human brain glioma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade; ICC/IF image of ab51997 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.