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Anti-CCR2 Antibody E68
Also for CCR2 (NM_001123041)
|A synthetic peptide corresponding to the N-terminal residues of the human C-C chemokine receptor type 2 was used as immunogen. The antibody does not cross-react with other CKR family members.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500 - 1:1000; FC: 1:25; IP: 1:50; IHC-Fr: Use at an assay dependent concentration; IHC-P: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Is unsuitable for ICC/IF.
|Homo sapiens chemokine (C-C motif) receptor 2 (CCR2), transcript variant A|
|CC-CKR-2; CCR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R|
Cytokine-cytokine receptor interaction
Signaling by GPCR
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Western blot - CCR2 antibody [E68]; All lanes : Anti-CCR2 [E68] antibody at 1/50 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : 1xPBS.Predicted band size : 42 kDa.Observed band size : 42 kDa.Additional bands at : 48 kDa,50 kDa (possible IgG),88 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - CCR2 antibody [E68]; ab32144, at a 1/100 dilution, staining human spleen by immunohistochemistry, Paraffin embedded tissue
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CCR2 antibody [E68]; ab32144 staining monkey spleen tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer pH 6.0 prior to blocking in 0.01% casein for 5 minutes at 20°C. The primary antbody was diluted 1/200 and incubated with the sample for 30 minutes at 20°C. A HRP-conjugated goat anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR2 antibody [E68]; IHC-P image of CCR2 staining on human tonsil tissue sections (1:2000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid pH 6. The sections were then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 1:2000 dilution for 2 hours at 21°C. Goat polyclonal to rabbit IgG conjugated to biotin was used as secondary (1:250).
Flow Cytometry - CCR2 antibody [E68]; ab32144 staining CCR2 in Human PBMC by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS; 2% BSA; 0.1% NaN3), for 1 hour at 4°C. An Alexa Fluor?-conjugated Goat polyclonal to Rabbit IgG (1/200) was used as secondary antibody. Gating strategy: Alive monocytes.