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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP859Y

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA303812 Rabbit monoclonal antibody against Histone H2b, Acetyl (K20)(EP859Y) 100ul $325 3-7 Days
LC422516 HIST2H2BF HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(6)
IF(1)
FC(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys 20 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 20.
Clone NameEP859Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:50000; IP: 1:10; FC: 1:10; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; ChIP: Use at an assay dependent dilution
Predicted MW Explanation 14 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Entrez Gene 440689 Human
Function
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
Western blot - Histone H2B (acetyl K20) antibody [EP859Y]; All lanes : Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade at 1/50000 dilution.Lane 1 : C6 cell lysate, untreated.Lane 2 : C6 cell lysate, treated with TSA.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 14 kDa.
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IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B (acetyl K20) antibody [EP859Y]; TA303812 at 1/250-1/500 dilution staining Histone H2B in human cervical carcinoma by Immunohistochemistry, Paraffin embedded tissue.
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IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade(TA303812); TA303812 showing positive staining in Normal tonsil tissue.
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IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade(TA303812); TA303812 showing positive staining in Ovarian carcinoma tissue.
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IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade(TA303812); TA303812 showing positive staining in Thyroid gland carcinoma tissue.
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IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade(TA303812); TA303812 showing positive staining in Breast carcinoma tissue.
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IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade(TA303812); TA303812 showing positive staining in Colonic adenocarcinoma tissue.
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IF Image
Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl K20) antibody [EP859Y]; ICC/IF image of TA303812 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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FC Image
Flow Cytometry-Anti-Histone H2B (acetyl K20) antibody [EP859Y] - ChIP Grade(TA303812); Overlay histogram showing HeLa cells stained with TA303812 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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