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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP857Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303808
  • Rabbit monoclonal antibody against Histone H2B, Acetyl (K5)(EP857Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC422516) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5.
Clone NameEP857Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:50000; CHIPseq: Use at an assay dependent concentration; ChIP: Use 6-25 ?g for ?g of chromatin; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Function
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H2B antibody [EP857Y]; All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade at 1/50000 dilution.Lane 1 : A431 cell lysate - untreated.Lane 2 : A431 cell lysate - treated with TSA.Lysates/proteins at 10 µg per lane.Predicted band size : 14 kDa.Observed band size : 14 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B antibody [EP857Y]; TA303808 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H2B antibody [EP857Y]; TA303808 (1:250) staining A431 cells by immunofluorescence.
10K-CHIP Image
ChIP - Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade; Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 6µl of TA303808 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
10K-CHIP Image
ChIP - Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade; TA303808 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.Detection step: Semiquantitative PCR.Negative control: Rabbit IgG.Cells treated with active vitamin D3.

 

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