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Anti-HIST2H2BF Antibody EP857Y
Also for HIST2H2BF (NM_001024599)
|A synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:50000; CHIPseq: Use at an assay dependent concentration; ChIP: Use 6-25 ?g for ?g of chromatin; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1|
|FLJ35099; FLJ56780; FLJ56787; MGC131639|
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Western blot - Histone H2B antibody [EP857Y]; All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade at 1/50000 dilution.Lane 1 : A431 cell lysate - untreated.Lane 2 : A431 cell lysate - treated with TSA.Lysates/proteins at 10 µg per lane.Predicted band size : 14 kDa.Observed band size : 14 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B antibody [EP857Y]; ab40886 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
Immunocytochemistry/ Immunofluorescence - Histone H2B antibody [EP857Y]; ab40886 (1:250) staining A431 cells by immunofluorescence.
ChIP - Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade; Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 6µl of ab40886 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade; ab40886 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4°C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.Detection step: Semiquantitative PCR.Negative control: Rabbit IgG.Cells treated with active vitamin D3.