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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP857Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303808
  • Rabbit monoclonal antibody against Histone H2B, Acetyl (K5)(EP857Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC422516) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(2)
IF(1)
Assay(2)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys 5 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lys 5.
Clone NameEP857Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Caenorhabditis elegans ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsWB: 1:50000; CHIPseq: Use at an assay dependent concentration; ChIP: Use 6-25 ?g for ?g of chromatin; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
Predicted MW Explanation 14 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Entrez Gene 440689 Human
Function
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H2B antibody [EP857Y]; All lanes : Anti-Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade at 1/50000 dilution.Lane 1 : A431 cell lysate - untreated.Lane 2 : A431 cell lysate - treated with TSA.Lysates/proteins at 10 ug per lane.Predicted band size : 14 kDa.Observed band size : 14 kDa.
Assay Image
ChIP - Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade; Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25ug of chromatin, 20ul of TA303808 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Assay Image
ChIP - Histone H2B (acetyl K5) antibody [EP857Y] - ChIP Grade; TA303808 at a 1/600 dilution for ChIP analysis of mouse dorsal skin epidermis whole tissue lysate, incubated for 15 hours at 4C with ChIP dilution buffer. Cross-linking (X-ChIP) using 1% formaldehyde for 10 minutes.Detection step: Semiquantitative PCR.Negative control: Rabbit IgG.Cells treated with active vitamin D3.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B antibody [EP857Y]; TA303808 staining human bladder carcinoma for Histone H2B expression (1:250 dilution)
IHC Image
Wholemount of Caenorhabditis elegans larvae labelling Histone H2B (acetyl K5) with TA303808. The sample was incubated with primary antibody (1/200 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000), was used as the secondary antibody.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H2B antibody [EP857Y]; TA303808 (1:250) staining A431 cells by immunofluorescence.

 

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