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Anti-HNRNPUL1 Antibody EP2633Y
Also for HNRNPUL1 (NM_007040)
|A synthetic peptide corresponding to residues on the C-terminus of human HNRPUL1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:50000; IP: 1:40; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens heterogeneous nuclear ribonucleoprotein U-like 1 (HNRNPUL1), transcript variant 1|
|E1B-AP5; E1BAP5; HNRPUL1|
|This gene encodes a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein binds specifically to adenovirus E1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus-infected cells. Two transcript variants encoding different isoforms have been found for this gene. Additional variants have also been found, but their full-length natures have not been determined. [provided by RefSeq]. |
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Western blot - HNRPUL1 antibody [EP2633Y]; Anti-HNRPUL1 antibody [EP2633Y] at 1/50000 dilution + HeLa cell lysate at 10 µg.Secondary.goat anti-rabbit, HRP conjugated at 1/2000 dilution.Predicted band size : 96 kDa.Observed band size : 96 kDa.Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HNRPUL1 antibody [EP2633Y]; ab68480 at 1/250 dilution staining HNRPUL1 in human brain by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry - HNRPUL1 antibody [EP2633Y]; ab68480 at 1/100 dilution staining HNRPUL1 in HeLa cells by Immunocytochemistry.
Flow Cytometry-Anti-HNRPUL1 antibody [EP2633Y](ab68480); Overlay histogram showing HeLa cells stained with ab68480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.