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Anti-BAZ1B Antibody EP1704Y
Also for BAZ1B (NM_032408)
|A synthetic peptide corresponding to residues near the C-terminus of human WSTF was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:15,000 - 20,000; IHC: 1:50 100; ICC: 1:100 250; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
* Availability is in business days
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Western blot - WSTF antibody [EP1704Y]; Anti-WSTF antibody [EP1704Y] at 1/20000 dilution + HeLa cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 171 kDa.Observed band size : 185 kDa .
Immunohistochemistry (Paraffin-embedded sections) - WSTF antibody [EP1704Y]; Ab51256 (1:50) staining human WSTF in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - WSTF antibody [EP1704Y]; Immunofluorescent staining of HeLa cells using TA303757 (1:100).
Flow Cytometry-Anti-WSTF antibody [EP1704Y](TA303757); Overlay histogram showing HeLa cells stained with TA303757 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.