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Anti-HIST3H3 Antibody Y28
Also for HIST3H3 (NM_003493)
|A synthetic acetylated peptide corresponding to residues surrounding Lys9 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 9.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IHC: 1:50 - 1:100; ICC: 1:250; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Homo sapiens histone cluster 3, H3 (HIST3H3)|
|H3.4; H3/g; H3FT; H3t|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
EGFR1 Signaling Pathway
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Western blot - Histone H3 antibody [Y28]; All lanes : Anti-Histone H3 (acetyl K9) antibody [Y28] at 1/500 dilution.Lane 1 : Untreated NIH 3T3 cell lysate.Lane 2 : NIH 3T3 cell lysate treated with TSA.Predicted band size : 15 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 antibody [Y28]; Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ab32129 at 1/50 - 1/100