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Home Antibody All anti-VAV2 antibodies

Anti-VAV2 Antibody EP1067Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303730
  • Rabbit monoclonal antibody against Vav 2 (EP1067Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418726) , 20ug
100ul 325 In Stock
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WB(1)
IP(1)
IHC(1)
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Also for VAV2 (NM_003371)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human Vav2 was used as an immunogen. This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.
Clone NameEP1067Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB: 1:20000; IP: Use at an assay dependent concentration; ICC: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens vav 2 guanine nucleotide exchange factor (VAV2), transcript variant 2
Alternative NameVAV-2
Database LinkNP_003362
FunctionVAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Signaling by GPCR

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - VAV2 antibody [EP1067Y]; Anti-VAV2 antibody [EP1067Y] at 1/20000 dilution + 293 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 101 kDa.Observed band size : 101 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - VAV2 antibody [EP1067Y]; Human cervical carcinoma stained with TA303730 at 1/50 - 1/100 dilution
IP Image
Immunoprecipitation - Anti-VAV2 antibody [EP1067Y]; VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA303730.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 100kDa; VAV2.

 

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