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Anti-ARPC5 Antibody EP1551Y
Also for ARPC5 (NM_005717)
|A synthetic peptide corresponding to residues on the N-terminus of human p16-ARC was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||ICC/IF: 1:50 - 1:100; FC: 1:50; IP: 1:70; WB: 1:20000; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
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Western blot - p16 ARC antibody [EP1551Y]; Anti-p16 ARC antibody [EP1551Y] at 1/20000 dilution + Fetal brain lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 16 kDa.
Immunohistochemistry (Paraffin-embedded sections) - p16 ARC antibody [EP1551Y]; Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.
Flow Cytometry - p16 ARC antibody [EP1551Y]; Overlay histogram showing HeLa cells stained with TA303704 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.