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Anti-SKP1 Antibody EPR3304
Also for SKP1 (NM_170679)
|A synthetic peptide corresponding to residues on human SKP1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; FC: 1:20; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens S-phase kinase-associated protein 1 (SKP1), transcript variant 2|
|EMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L|
|This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq]. |
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Skp1 antibody [EPR3304]; All lanes : Anti-Skp1 antibody [EPR3304] at 1/2000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC-12 cell lysate.Lane 4 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labeled IgG at 1/2000 dilution.Predicted band size : 19 kDa.Observed band size : 19 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-Skp1 antibody [EPR3304]; ab76502 staining Skp1 in HeLa cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, blocked with 1% BSA for 30 minutes at 24°C then incubated with ab76502 at a 1/500 dilution for 1 hour at 24°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.