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Home Antibody All anti-SKP1 antibodies

Anti-SKP1 Antibody EPR3304

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303696
  • Rabbit monoclonal antibody against SKP1 (EPR3304 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC406907) , 20ug
100uL 325 3-7 Days
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WB(1)
IF(1)
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Also for SKP1 (NM_170679)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on human SKP1 was used as an immunogen.
Clone NameEPR3304 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:1000 - 1:10000; FC: 1:20; ICC/IF: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens S-phase kinase-associated protein 1 (SKP1), transcript variant 2
Alternative NameEMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L
Database LinkNP_733779
FunctionThis gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq].
Related Pathway
Cell cycle
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - Skp1 antibody [EPR3304]; All lanes : Anti-Skp1 antibody [EPR3304] at 1/2000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC-12 cell lysate.Lane 4 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labeled IgG at 1/2000 dilution.Predicted band size : 19 kDa.Observed band size : 19 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Skp1 antibody [EPR3304]; TA303696 staining Skp1 in HeLa cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol, blocked with 1% BSA for 30 minutes at 24°C then incubated with TA303696 at a 1/500 dilution for 1 hour at 24°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

 

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