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Home Antibody All anti-RB1 antibodies

Anti-RB1 PHOSPHO Antibody EP821Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303684
  • Rabbit monoclonal antibody against Phospho-Rb (pT373)(EP821Y) (phospho-specific)
100ul $325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr373 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 373.
Clone NameEP821Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:50,000; IHC: 1:2501:500; ICC: 1:50 100; IP: 1:30
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens retinoblastoma 1 (RB1)
Alternative NameOSRC; p105-Rb; pp110; pRb; RB
Database LinkNP_000312
FunctionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq].
Related Pathway
Adipogenesis
Cell cycle
Senescence and Autophagy
TGF Beta Signaling Pathway

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WB Image
Western blot - Rb (phospho T373) antibody [EP821Y]; All lanes : Anti-Rb (phospho T373) antibody [EP821Y] at 1/50000 dilution.Lane 1 : Jurkat cell lysate (untreated).Lane 2 : Jurkat cell lysate (treated with FBS).Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 110 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Rb (phospho T373) antibody [EP821Y]; TA303684, at a 1/250 dilution, staining Human Rb (Phosphorylated) in Kidney carcinoma, using Immunohistochemistry, Paraffin Embedded tissue.

 

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