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Home Antibody All anti-RB1 antibodies

Anti-RB1 PHOSPHO Antibody EP821Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303684
  • Rabbit monoclonal antibody against Phospho-Rb (pT373)(EP821Y) (phospho-specific)
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr373 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 373.
Clone NameEP821Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:50,000; IHC: 1:2501:500; ICC: 1:50 100; IP: 1:30
Predicted MW Explanation 106 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens retinoblastoma 1 (RB1)
Alternative NameOSRC; p105-Rb; pp110; pRb; RB
Database LinkNP_000312
Entrez Gene 5925 Human
FunctionThe protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq].
Related PathwayTranscription FactorsDruggable Genome Cell cyclePathways in cancerPancreatic cancerGliomaProstate cancerMelanomaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Rb (phospho T373) antibody [EP821Y]; All lanes : Anti-Rb (phospho T373) antibody [EP821Y] at 1/50000 dilution.Lane 1 : Jurkat cell lysate (untreated).Lane 2 : Jurkat cell lysate (treated with FBS).Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 110 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Rb (phospho T373) antibody [EP821Y]; TA303684, at a 1/250 dilution, staining Human Rb (Phosphorylated) in Kidney carcinoma, using Immunohistochemistry, Paraffin Embedded tissue.

 

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