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Anti-RB1 PHOSPHO Antibody EP821Y
|A synthetic phospho-peptide corresponding to residues surrounding Thr373 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 373.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50,000; IHC: 1:2501:500; ICC: 1:50 100; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens retinoblastoma 1 (RB1)|
|OSRC; p105-Rb; pp110; pRb; RB|
|The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]. |
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Western blot - Rb (phospho T373) antibody [EP821Y]; All lanes : Anti-Rb (phospho T373) antibody [EP821Y] at 1/50000 dilution.Lane 1 : Jurkat cell lysate (untreated).Lane 2 : Jurkat cell lysate (treated with FBS).Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 106 kDa.Observed band size : 110 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Rb (phospho T373) antibody [EP821Y]; TA303684, at a 1/250 dilution, staining Human Rb (Phosphorylated) in Kidney carcinoma, using Immunohistochemistry, Paraffin Embedded tissue.