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Anti-RB1 PHOSPHO Antibody E231
|A synthetic phospho-peptide corresponding to residues surrounding Thr821 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Threonine 821.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; ICC/IF: 1:100; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
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Western blot - Rb (phospho T821) antibody [E231]; All lanes : Anti-Rb (phospho T821) antibody [E231] at 1/1000 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate treated with Phosphatase.Predicted band size : 106 kDa.Observed band size : 110 kDa .
Immunofluorescence - Rb (phospho T821) antibody [E231]; Ab32015, at a 1/100 dilution, staining Rb in HeLa cells by Immunofluorescence.