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Anti-RB1 PHOSPHO Antibody E182
|A synthetic phospho-peptide corresponding to residues surrounding Ser780 of human Rb was used as immunogen. The antibody only detects Rb phosphorylated on Serine 780.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500 - 1000; ICC: 1:250-1:500; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
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Western blot - Rb (phospho S780) antibody [E182]; All lanes : Anti-Rb [E182] antibody at 1/1000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with FBS.Lane 3 : Jurkat cell lysate treated with Alkaline Phosphatase.Predicted band size : 106 kDa.Observed band size : 110 kDa .
Immunocytochemistry/ Immunofluorescence - Rb (phospho S780) antibody [E182]; Immunofluorescent staining of (A) non-treated and (B) Lambda Phosphatase-treated A431 cells using anti-Phospho-Rb (S780) TA303682 Rabbit Monoclonal Antibody