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Anti-MAPK1 Antibody E460
Also for MAPK1 (NM_002745)
|A synthetic peptide corresponding to residues near the C-term of human ERK2 p42 MAPK was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:2000; IHC-P: Use at an assay dependent concentration; ICC: 1:100 - 1:250; FC: 1:1000; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens mitogen-activated protein kinase 1 (MAPK1), transcript variant 1|
|ERK; ERK2; ERT1; MAPK2; p38; p40; p41; p41mapk; P42MAPK; PRKM1; PRKM2|
|The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]. |
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
Senescence and Autophagy
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - ERK2 antibody [E460]; Anti-ERK2 antibody [E460] at 1/2000 dilution + A431 cell lysate.Predicted band size : 41 kDa.Observed band size : 41 kDa.
Immunohistochemistry (Paraffin-embedded sections) - ERK2 antibody [E460]; Immunohistochemical analysis of ERK2 expression in paraffin embedded human breast carcinoma tissue section, using at a dilution of 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody [E460]; Immunohistochemical analysis of Human fallopian tissue epithelium, staining ERK2 with ab32081 at 1/50 dilution. Samples were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
Flow Cytometry - Anti-ERK2 antibody [E460]; Overlay histogram showing HeLa cells stained with ab32081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.