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Anti-PHB Antibody EP2804Y
|A synthetic peptide corresponding to residues in human prohibitin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC-Fr: Use at an assay dependent concentration; WB: 1:500 - 1:1000; IP: 1:20; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens prohibitin (PHB), transcript variant 2|
|Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq]. |
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Western blot - Prohibitin antibody [EP2804Y]; All lanes : Anti-Prohibitin antibody [EP2804Y] at 1/1000 dilution.Lane 1 : MCF-7 cell lysates.Lane 2 : PC-12 cell lysates.Lane 3 : A431 cell lysates.Lane 4 : Ramos cell lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.Observed band size : 30 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Prohibitin antibody [EP2804Y]; ab75771 at 1/100 dilution staining Prohibitin in human hepatocellular carcinoma tissue, using a HRP/AP polymerized secondary antibody.
Immunocytochemistry/ Immunofluorescence - Prohibitin antibody [EP2804Y]; Immunofluorescent staining of HeLa cells using ab75771 at a 1/100 dilution.