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Anti-NFKB1 Antibody E381
Also for NFKB1 (NM_003998)
|A synthetic peptide corresponding to residues in the nuclear factor NF-?B p50 subunit was used as immunogen. This antibody will detect both forms; p50 and p105.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Is unsuitable for IP.
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Western blot - NFkB p105 / p50 antibody [E381]; Anti-NFkB p105 / p50 antibody [E381] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 50 kDa.Observed band size : 50,105 kDa .
Immunohistochemistry (Paraffin-embedded sections) - NFkB p105 / p50 antibody [E381]; Paraffin-embedded human prostate carcinoma.TA303617 at 1/250 dilution
Immunocytochemistry/ Immunofluorescence - NFkB p105 / p50 antibody [E381]; ICC/IF image of TA303617 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-NFkB p105 / p50 antibody [E381](TA303617); Overlay histogram showing K562 cells stained with TA303617 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.