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Home Antibody All anti-NFE2L2 antibodies

Anti-NFE2L2 PHOSPHO Antibody EP1809Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303614
  • Rabbit monoclonal antibody against Nrf2 (pS40) Phospho (EP1809Y ) (Phospho-Specific)
100ul 325 In Stock
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WB(1)
IHC(6)
IF(1)
FC(1)
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Also for NFE2L2 (NM_006164)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the serine 40 of human Nrf2 protein.
Clone NameEP1809Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: Use a concentration of 1 ug/ml; IHC-P: 1:100 - 1:250; WB: 1:5000 - 1:50000; IP: 1:40; ICC: 1:100 - 1:250; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens nuclear factor, erythroid 2-like 2 (NFE2L2), transcript variant 1
Alternative NameNRF2
Database LinkNP_006155
FunctionNFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Nrf2 (phospho S40) antibody [EP1809Y]; All lanes : Anti-Nrf2 (phospho S40) antibody [EP1809Y] at 1/20000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : HepG2 cell lysate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 68 kDa.Observed band size : 90 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA303615 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 (phospho S40) antibody [EP1809Y]; TA303615 staining Nrf2 (phospho S40) in Human normal lung tissue sections by IHC-P (Formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% casein for 30 minutes at 4°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) in 1% casein for 24 hours at 4°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as the secondary antibody.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](TA303615); TA303615 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](TA303615); TA303615 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](TA303615); TA303615 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Nrf2 (phospho S40) antibody [EP1809Y](TA303615); TA303615 showing positive staining in Normal tonsil tissue.
IF Image
Immunocytochemistry/ Immunofluorescence-Nrf2 (phospho S40) antibody [EP1809Y](TA303615); ICC/IF image of TA303615 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
FC Image
Flow Cytometry - Anti-Nrf2 (phospho S40) antibody [EP1809Y]; Overlay histogram showing Jurkat cells stained with TA303615 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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