OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-NCK1 antibodies

Anti-NCK1 Antibody Y531

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303611
  • Rabbit monoclonal antibody against Nck(Y531)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416832) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.
Clone NameY531 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000 - 1:10000; IHC-P: Use at an assay dependent concentration; ICC: 1:250 - 1:500; FC: 1:1000; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction

Reference Data

Target NameHomo sapiens NCK adaptor protein 1 (NCK1), transcript variant 1
Alternative NameNCK; nck-1; NCKalpha
Database LinkNP_006144
FunctionThe protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Nck antibody [Y531]; Anti-Nck antibody [Y531] at 1/10000 dilution + Hela cell lysate.Observed band size : 47 kDa .Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Nck antibody [Y531]; TA303611, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
FC Image
Flow Cytometry - Anti-Nck antibody [Y531]; Overlay histogram showing Jurkat cells stained with TA303611 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title