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Home Antibody All anti-MST1R antibodies

Anti-MST1R Antibody EP1132Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303606
  • Rabbit monoclonal antibody against Ron(EP1132Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419321) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Ron was used as an immunogen.
Clone NameEP1132Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:10000; IP: 1:30; FC: 1:20 - 1:40; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R), transcript variant 1
Alternative NameCD136; CDw136; PTK8; RON
Database LinkNP_002438
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RON antibody [EP1132Y]; Anti-RON antibody [EP1132Y] at 1/10000 dilution + SKBR3 cell lysate at 10 ug.Secondary.HRP-labelled goat anti-rabbit.Predicted band size : 185 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - RON antibody [EP1132Y]; Immunohistochemical staining of paraffin-embedded human stomach tissue using TA303606 at a dilution of 1/100-1/250.
FC Image
Flow Cytometry - RON antibody [EP1132Y]; Overlay histogram showing THP1 cells stained with TA303606 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.

 

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