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Anti-MST1R Antibody EP1132Y
Also for MST1R (NM_002447)
|A synthetic peptide corresponding to residues near the N-terminus of human Ron was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:10000; IP: 1:30; FC: 1:20 - 1:40; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens macrophage stimulating 1 receptor (c-met-related tyrosine kinase) (MST1R), transcript variant 1|
|CD136; CDw136; PTK8; RON|
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Western blot - RON antibody [EP1132Y]; Anti-RON antibody [EP1132Y] at 1/10000 dilution + SKBR3 cell lysate at 10 µg.Secondary.HRP-labelled goat anti-rabbit.Predicted band size : 185 kDa.
Immunohistochemistry (Paraffin-embedded sections) - RON antibody [EP1132Y]; Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250.
Flow Cytometry - RON antibody [EP1132Y]; Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.