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Home Antibody All anti-MBP antibodies

Anti-MBP Antibody EP1448Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303591
  • Rabbit monoclonal antibody against MBP (EP1448Y )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC422582) , 20ug
100ul 325 In Stock
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WB(1)
IF(1)
FC(1)
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Also for MBP (NM_001025090)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues of human MBP was used as an immunogen.
Clone NameEP1448Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; FC: 1:60 - 1:1000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens myelin basic protein (MBP), transcript variant 3
Alternative NameMGC99675
Database LinkNP_001020261
FunctionThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq].
Related Pathway

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WB Image
Western blot - Myelin Basic Protein antibody [EP1448Y]; Anti-Myelin Basic Protein antibody [EP1448Y] at 1/5000 dilution + Fetal brain at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 33 kDa.Observed band size : 33 kDa.
IF Image
Immunocytochemistry - Myelin Basic Protein antibody [EP1448Y]; PC12 cells labelled with TA303591 at 1/100 - 1/250 dilution
FC Image
Flow Cytometry - Anti-Myelin Basic Protein antibody [EP1448Y]; Overlay histogram showing SH-SY5Y cells stained with TA303591 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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