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Anti-MBP Antibody EP1448Y
Also for MBP (NM_001025090)
|A synthetic peptide corresponding to residues of human MBP was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; ICC: 1:100 - 1:250; FC: 1:60 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens myelin basic protein (MBP), transcript variant 3|
|The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq]. |
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Western blot - Myelin Basic Protein antibody [EP1448Y]; Anti-Myelin Basic Protein antibody [EP1448Y] at 1/5000 dilution + Fetal brain at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 33 kDa.Observed band size : 33 kDa.
Immunocytochemistry - Myelin Basic Protein antibody [EP1448Y]; PC12 cells labelled with ab53294 at 1/100 - 1/250 dilution
Flow Cytometry - Anti-Myelin Basic Protein antibody [EP1448Y]; Overlay histogram showing SH-SY5Y cells stained with ab53294 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.