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Anti-MARCKS PHOSPHO Antibody EP2113Y
Also for MARCKS (NM_002356)
|A synthetic phospho-peptide corresponding to residues surrounding serine 159 and 163 of human MARCKS protein. The orthologous phosphorylation sites in mouse and rat is serine 152 and 156|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||IP: 1:40; WB: 1:1000 - 1:2000
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens myristoylated alanine-rich protein kinase C substrate (MARCKS)|
|80K-L; MACS; PKCSL; PRKCSL|
|The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq]. |
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Western blot - Anti-MARCKS (phospho S158) antibody [EP2113Y]; All lanes : Anti-MARCKS (phospho S158) antibody [EP2113Y] at 1/2000 dilution.Lane 1 : Hela cell lysates, untreated.Lane 2 : Hela cell lysates treated with TPA.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit at 1/2000 dilution.Predicted band size : 32 kDa.Observed band size : 87 kDa .