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Anti-ITGAM Antibody EP1345Y
Also for ITGAM (NM_000632)
|A synthetic peptide corresponding to C-terminus of human CD11b/ITAM protein was used as immunogen|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||IHC-FoFr: Use at an assay dependent concentration; WB: 1:20000 - 1:50000; IP: 1:80; ICC: 1:100 - 1:250; FC: 1:20 - 1:50; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2|
|CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6|
|This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Some integrin alpha M proteins contain an inserted amino acid (Q, between AA positions 499 and 500) resulting from a 3bp shift in a splice acceptor that inserts the codon CAG; receptors with or without the extra amino acid are functionally indistinguishable [provided by RefSeq]. |
Hematopoietic cell lineage
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Western blot - CD11b antibody [EP1345Y]; Anti-CD11b antibody [EP1345Y] at 1/20000 dilution + TF1 lysate at 10 ug.Secondary.goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 128 kDa.Observed band size : 170 kDa .
Western blot - Anti-CD11b antibody [EP1345Y]; All lanes : Anti-CD11b antibody [EP1345Y] at 1/5000 dilution.Lane 1 : THP-1 monocytes, untreated.Lane 2 : THP-1 monocytes, +10 ng/ml LPS.Lane 3 : THP-1 macrophages, untreated.Lane 4 : THP-1 macrophages, +10 ng/ml LPS.Lysates/proteins at 40 ug per lane.Secondary.IRDye680-conjugated donkey anti-rabbit IgG.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 128 kDa.Observed band size : 180 kDa .Exposure time : 4 minutes
Immunohistochemistry (Paraffin-embedded sections) - CD11b antibody [EP1345Y]; Immunohistochemical analysis of paraffin-embedded human spleen using TA303553 at a dilution of 1/100.
Flow Cytometry-CD11b antibody [EP1345Y](TA303553); Overlay histogram showing RAW 264.7 cells stained with TA303553 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.