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Anti-INSR PHOSPHO Antibody EP351(2)Y
Also for INSR (NM_000208)
|A phospho synthetic peptide corresponding to residues surrounding Tyrosine 1185 of human INSR was used as an immunogen. This antibody detects INSR phosphorylated at Tyrosine 1185.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000; IP: 1:20; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IHC-P.
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Western blot - Insulin Receptor (phospho Y1185) antibody [EP351(2)Y]; All lanes : Anti-Insulin Receptor (phospho Y1185) antibody [EP351(2)Y] at 1/500 dilution.Lane 1 : HepG2 cell lysate; untreated.Lane 2 : HepG2 cell lysate; Insulin treated.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP conjugated, at 1/2000 dilution.Predicted band size : 156 kDa.Observed band size : 95 kDa .