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Home Antibody All anti-IHH antibodies

Anti-IHH Antibody EP1192Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303543
  • Rabbit monoclonal antibody against Indian Hedgehog (IHH) (EP1192Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419483) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human IHH was used as an immunogen.
Clone NameEP1192Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:10000; IP: 1:30; IHC-P: Use at an assay dependent dilution; IHC-FoFr: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens indian hedgehog (IHH)
Alternative NameBDA1; HHG2
Database LinkNP_002172
Function
Related Pathway
Hedgehog signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ihh antibody [EP1192Y]; Anti-Ihh antibody [EP1192Y] at 1/10000 dilution + Human fetal brain lysate at 10 ug.Secondary.Goat anti rabbit hrp conjugated at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Ihh antibody [EP1192Y]; Ab52919 at 1/250 dilution staining human fetal colon tissue; paraffin embedded.
IHC Image
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y]; Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with TA303543. Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5?-conjugated goat anti-rabbit IgG as secondary antibody.

 

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