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Anti-IHH Antibody EP1192Y
|A synthetic peptide corresponding to residues near the N-terminus of human IHH was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000; IP: 1:30; IHC-P: Use at an assay dependent dilution; IHC-FoFr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens indian hedgehog (IHH)|
Hedgehog signaling pathway
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Western blot - Ihh antibody [EP1192Y]; Anti-Ihh antibody [EP1192Y] at 1/10000 dilution + Human fetal brain lysate at 10 µg.Secondary.Goat anti rabbit hrp conjugated at 1/2000 dilution.Predicted band size : 45 kDa.Observed band size : 45 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Ihh antibody [EP1192Y]; Ab52919 at 1/250 dilution staining human fetal colon tissue; paraffin embedded.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Ihh antibody [EP1192Y]; Immunohistochemical analysis of murine intervertebral discs, staining Ihh (red) with ab52919. Sections were fixed in paraformaldehyde, permeabilized using 0.2% Triton X-100 for 20 minutes and blocked in blocking buffer for one hour. Samples were incubated with diluted primary antibody overnight at 4°C before being incubated with a Cy5?-conjugated goat anti-rabbit IgG as secondary antibody.