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Home Antibody All anti-HSF1 antibodies

Anti-HSF1 PHOSPHO Antibody EP1713Y

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SKU Description Amount Price Availability*  
TA303540 Rabbit monoclonal antibody against HSF1 Phospho (pS326) (EP1713Y ) (phospho-specific) 100ul $325 In Stock

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WB(1)
IHC(2)
FC(1)

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Ser326 of human HSF1 was used as an immunogen. This antibody detects HSF1 phosphorylated on Ser326.
Clone NameEP1713Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000 - 1:10000; IP: 1:80; ICC: 1:250 - 1:500; FC: 1:1000; IHC-P: 1:100 - 1:250
Predicted MW Explanation 57 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens heat shock transcription factor 1 (HSF1)
Alternative NameHSTF1
Database LinkNP_005517
Entrez Gene 3297 Human
FunctionThe product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq].
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - HSF1 (phospho S326) antibody [EP1713Y]; All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] at 1/10000 dilution.Lane 1 : HeLa cell lysates (untreated).Lane 2 : HeLa cell lysates, treated with heat (44o C).Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 57 kDa.Observed band size : 82 kDa .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S326) antibody [EP1713Y]; Immunohistochemical analysis of paraffin-embedded human stomach using TA303540, at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S326) antibody [EP1713Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using TA303540, at 1/100 dilution.
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Flow Cytometry - Anti-HSF1 (phospho S326) antibody [EP1713Y]; Overlay histogram showing HeLa cells stained with TA303540 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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