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Anti-HSF1 PHOSPHO Antibody EP1713Y
Also for HSF1 (NM_005526)
|A phospho specific peptide corresponding to residues surrounding Ser326 of human HSF1 was used as an immunogen. This antibody detects HSF1 phosphorylated on Ser326.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000 - 1:10000; IP: 1:80; ICC: 1:250 - 1:500; FC: 1:1000; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens heat shock transcription factor 1 (HSF1)|
|The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq]. |
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Western blot - HSF1 (phospho S326) antibody [EP1713Y]; All lanes : Anti-HSF1 (phospho S326) antibody [EP1713Y] at 1/10000 dilution.Lane 1 : HeLa cell lysates (untreated).Lane 2 : HeLa cell lysates, treated with heat (44o C).Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 57 kDa.Observed band size : 82 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S326) antibody [EP1713Y]; Immunohistochemical analysis of paraffin-embedded human stomach using ab76076, at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HSF1 (phospho S326) antibody [EP1713Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ab76076, at 1/100 dilution.
Flow Cytometry - Anti-HSF1 (phospho S326) antibody [EP1713Y]; Overlay histogram showing HeLa cells stained with ab76076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.