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Home Antibody All anti-HSF1 antibodies

Anti-HSF1 Antibody EP1710Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303539
  • Rabbit monoclonal antibody against HSF1 (EP1710Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417243) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for HSF1 (NM_005526)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human HSF1 was used as an immunogen.
Clone NameEP1710Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:100000; IP: 1:100; FC: 1:1000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens heat shock transcription factor 1 (HSF1)
Alternative NameHSTF1
Database LinkNP_005517
Entrez Gene 3297 Human
FunctionThe product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq].
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HSF1 antibody [EP1710Y]; Anti-HSF1 antibody [EP1710Y] at 1/100000 dilution + Hela cell lysate at 10 ug.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 57 kDa.Observed band size : 85 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - HSF1 antibody [EP1710Y]; Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using TA303539 at a 1:100 dilution.
FC Image
Flow Cytometry - Anti-HSF1 antibody [EP1710Y]; Overlay histogram showing HeLa cells stained with TA303539 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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