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Anti-FLT1 Antibody Y103
Also for FLT1 (NM_002019)
|A synthetic peptide corresponding to residues in N-terminus of human VEGFR1 was used as immunogen. The antibody can detect both VEGFR1 and its splice isoform sFlt1.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:1000 - 1:5000; IHC-P: 1:250; ICC/IF: 1:250; IP: 1:100; FC: 1:1000; IHC-Fr: 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - VEGF Receptor 1 antibody [Y103]; Anti-VEGF Receptor 1 antibody [Y103] at 1/10000 dilution + mouse brain tissue.Predicted band size : 151 kDa.Observed band size : 180 kDa .
Immunohistochemistry (Paraffin-embedded sections) - VEGF Receptor 1 antibody [Y103]; Immunohistochemical analysis of VEGF receptor 1 expression in paraffin-embedded skin cancer, using 1/250 TA303515.
Immunocytochemistry/ Immunofluorescence - VEGF Receptor 1 antibody [Y103]; Immunofluorescent analysis of VEGF receptor 1 expression in A431 cells using 1/250 TA303515.
Immunocytochemistry/ Immunofluorescence - VEGF Receptor 1 antibody [Y103]; TA303515 at 1/200 dilution staining VEGF Receptor 1 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25Â°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4Â°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution.
Flow Cytometry - Anti-VEGF Receptor 1 antibody [Y103]; Overlay histogram showing A431 cells stained with TA303515 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.