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Anti-ERBB4 PHOSPHO Antibody EP2270Y
Also for ERBB4 (NM_005235)
|A phospho specific peptide corresponding to residues surrounding tyrosine 1162 of human Her4/ErbB-4 was used as an immunogen. This antibody detects Her4/ErbB-4 phosphorylated on tyrosine 1162.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:25,000; IHC: 1:100; IP: 1:30
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4 (ERBB4), transcript variant JM-a/CVT-1|
|ALS19; HER4; p180erbB4|
Entrez Gene 2066 Human
|This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]. |
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Western blot - ErbB 4 (phospho Y1162) antibody [EP2270Y]; All lanes : Anti-ErbB 4 (phospho Y1162) antibody [EP2270Y] at 1/500 dilution.Lane 1 : A431 cell lysate. Cells untreated.Lane 2 : A431 cell lysate. Cells treated with EGF.Lysates/proteins at 10 ug per lane.Secondary.HRP conjugated Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 147 kDa.Observed band size : 200 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ErbB 4 (phospho Y1162) antibody [EP2270Y]; Human breast carcinoma stained with TA303503 at 1/100 dilution.