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Anti-BTK PHOSPHO Antibody EP420Y
|A phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IP: 1:40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IHC-P.
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Western blot - BTK (phospho Y223) antibody [EP420Y]; All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] at 1/500000 dilution.Lane 1 : Ramos cell lysates, cells un-treated.Lane 2 : Ramos cell lysates, cells treated with pervanadate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 76 kDa.Observed band size : 76 kDa.