A phospho specific peptide corresponding to residues surrounding tyrosine 223 of human BTK was used as an immunogen. This antibody detects BTK phosphorylated on tyrosine 223.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Clone Name
EP420Y
Isotype
Rabbit IgG
Species Reactivity
Human
Concentration
Purification
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:100,000 - 500,000 IP: 1:40
Background
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq].
Related Pathway
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Western blot analysis on Ramos cell lysates using anti-Phospho-BTK (pY223) RabMAb (TA303406), 1:500,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
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