OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-BCL2A1 antibodies

Anti-BCL2A1 Antibody EP517Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303401
  • Rabbit monoclonal antibody against Bcl-2 related protein A1/Bfl1 (N-term) (EP517Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418253) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IHC(1)
IF(1)
FC(1)
spacer
Also for BCL2A1 (NM_004049)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-term of human Bcl-2 related protein A1 was used as immunogen.
Clone NameEP517Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:500; IHC-P: Use at an assay dependent dilution; FC: 1:100
Predicted MW Explanation 20 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification IgG fraction
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens BCL2-related protein A1 (BCL2A1), transcript variant 1
Alternative NameACC-1; ACC-2; BCL2L5; BFL1; GRS; HBPA1
Database LinkNP_004040
Entrez Gene 597 Human
Entrez Gene 170929 Rat
FunctionThis gene encodes a member of the BCL-2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and is up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayDruggable Genome Metabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - A1 antibody [EP517Y]; Anti-Bcl2A1 antibody [EP517Y] at 1/500 dilution + Raji cell lysate.Predicted band size : 20 kDa.Observed band size : 27 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - A1 antibody [EP517Y]; TA303401, at a 1/50 dilution, staining human adenocarcinoma by Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Bcl2A1 antibody [EP517Y]; TA303401 at 1/100, staining Hela cell lysate by Immunofluorescent.
FC Image
Flow Cytometry - Bcl2A1 antibody [EP517Y]; Overlay histogram showing HepG2 cells stained with TA303401 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title