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Anti-APAF1 Antibody E38
Also for APAF1 (NM_001160)
|A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100
|Does not react with Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or IP.
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Western blot - APAF1 antibody [E38]; Anti-APAF1 antibody [E38] at 1/500 dilution + HeLa cell lysate.Predicted band size : 141 kDa.Observed band size : 135 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Cat# TA303393 - APAF1 antibody [E38] at a dilution of 1/50, staining APAF1 in paraffin embedded stomach adenocarcinoma by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-APAF1 antibody [E38]; TA303393 staining APAF1 in human leukocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, permeabilized using 0.3% Triton X-100, blocked with 2% serum for 2 hours at 25°C, and then incubated with TA303393 at a 1/250 dilution for 9 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.