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Anti-ALK PHOSPHO Antibody EP661Y
|A synthetic phosphor-peptide corresponding to residues surrounding Tyr1604 of human ALK was used as an immunogen. The antibody only detects ALK phosphorylated on Tyrosine 1604.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:5000; IP: 1:20; IHC-P: Use at an assay dependent dilution; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens anaplastic lymphoma receptor tyrosine kinase (ALK)|
|The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq]. |
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Western blot - ALK (phospho Y1604) antibody [EP661Y]; All lanes : Anti-ALK (phospho Y1604) antibody [EP661Y] at 1/5000 dilution.Lane 1 : SH-SY-5Y cell lysate, untreated.Lane 2 : SH-SY-5Y cell lysate, treated with PTN.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 176 kDa.Observed band size : 220 kDa .
Immunohistochemistry (Paraffin-embedded sections) - ALK (phospho Y1604) antibody [EP661Y]; Ab51030 (1:100) staining human ALK in human Non-Hodgkins lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
Flow Cytometry-Anti-ALK (phospho Y1604) antibody [EP661Y](ab51030); Overlay histogram showing SH-SY5Y cells stained with ab51030 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.