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Anti-PARP1 Antibody E102
Also for PARP1 (NM_001618)
|A synthetic peptide corresponding to N-terminal residues of human PARP-1 was used as immunogen. The antibody should recognize both pro-form and p25 cleaved-form of PARP-1.|
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:500 - 1,000; IHC: 1:25; ICC: 1:100; FC: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens poly (ADP-ribose) polymerase 1 (PARP1)|
|ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL|
|This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq]. |
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All lanes : Anti-PARP antibody [E102] (TA303384) at 1/1000 dilution. Lane 1 : Jurkat cell lysate; Lane 2 : Jurkat + Camptothecin cell lysate.
Immunohistochemical analysis of PARP expression in paraffin embedded human brain tissue section, using 1/25 TA303384.
TA303384 (1/200) staining PARP in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red).
Overlay histogram showing Jurkat cells stained with TA303384 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (TA303384, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.