Homo sapiens ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) (RAC2)
Synonyms:
EN-7; Gx; HSPC022
Immunogen
Peptide with sequence PQPTRQQKRACSLL, from the C Terminus of the protein sequence according to NP_002863.1.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clone Name
Isotype
Goat IgG
Species Reactivity
Human
Concentration
0.5 mg/ml
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
ELISA: 1:32,000. WB: 1-3µg/ml. IHC: 0.3ug/ml.
Background
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. [provided by RefSeq].
Related Pathway
Adherens junction
Focal Adhesion
MAPK signaling pathway
Signaling by GPCR
Wnt Signaling Pathway
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TA302763 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA302763retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.
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