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Home Antibody All anti-PPP1R15A antibodies

Anti-PPP1R15A Antibody

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA302708 Goat Polyclonal Antibody against PPP1R15A 100ug $325 3-7 Days
LC415358 PPP1R15A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(1)
IF(2)
FC(1)
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Also for PPP1R15A (NM_014330)
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OriGene Data

ImmunogenPeptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.2.
Clone Name IsotypeGoat IgG
Species ReactivityTest: Human, Mouse. Expected from seq similarity: Human Concentration0.5 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsELISA: 1:128,000. WB: 0.1-0.3µg/ml.
BufferSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Reference Data

Target Nameprotein phosphatase 1 regulatory subunit 15A
Alternative NameGADD34
Database LinkNP_055145
Entrez Gene 23645 Human
Entrez Gene 17872 Mouse
FunctionThis gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq].
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
TA302708(0.3µg/ml) staining of HepG2 (A) and negative control KLY (B) lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
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IHC Image
TA302708(2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
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IF Image
TA302708 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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IF Image
TA302708 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
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FC Image
TA302708 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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