Peptide with sequence C-RELGEYGFHEYTE, from the internal region of the protein sequence according to NP_000680.2.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clone Name
Isotype
Goat IgG
Species Reactivity
Tested: Human Expected from sequence similarity: Human, Mouse, Rat
Concentration
0.5 mg/ml
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Guaranteed Application *
WB
Suggested Dilutions
Peptide ELISA: antibody detection limit dilution 1:32,000. Western blot recommended concentration: 1-3µg/ml.
Background
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq].
HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY ALDH1A1 (RC200723) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1A1.
