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Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (N-term) (Cat.#TA302271). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
CD14 Antibody (N-term) (Cat.#TA302271) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (N-term) (Cat.#TA302271), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of CD14 Antibody (N-term)(Cat#TA302271) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
CD14 Antibody (N-term) (Cat. #TA302271) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.