Anti-FRAP1 Antibody,

Also for FRAP1 (NM_004958)

OriGene Data

Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S2481 of human FRAP1.
Clone Name		Isotype	Rabbit Ig
Species Reactivity	Human	Concentration	0.25 mg/ml
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
Buffer	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification	Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)
Alternative Name	FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Database Link	NP_004949
Function	FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
Related Pathway	Senescence and Autophagy

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WB Image

Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies).

IHC Image

Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-FRAP1 Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data