Home Antibody All anti-PTPN11 antibodies
Also for PTPN11 (NM_002834)
|This SHP2 antibody is generated from rabbits immunized with a recombinant protein of partial human SHP2.|
||IF: 1:10~50, WB: 1:1000
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
|Homo sapiens protein tyrosine phosphatase, non-receptor type 11 (PTPN11), transcript variant 1|
|BPTP3; CFC; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2|
Entrez Gene 5781 Human
|SHP2, also known as PTPN11, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in the gene are a cause of Noonan syndrome as well as acute myeloid leukemia.|
|PhosphataseDruggable Genome Jak-STAT signaling pathwayNatural killer cell mediated cytotoxicityLeukocyte transendothelial migrationNeurotrophin signaling pathwayAdipocytokine signaling pathwayEpithelial cell signaling in Helicobacter pylori infectionMore Pathways >> |
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Western blot analysis of PTPN11 (arrow) using rabbit polyclonal SHP2 Antibody (RB07283). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN11 gene (Lane 2) (Origene Technologies).
Fluorescent image of Hela cell stained with SHP2 Antibody(Cat#TA302214/SH050329B).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SHP2 primary antibody (1:25, 1 h at 37Ã¢). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37Ã¢).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37Ã¢).SHP2 immunoreactivity is localized to Nucleolus and Cytoplasm significantly and Nucleus weakly.