This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S424 of human RPS6KB1.
Buffer
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clone Name
Isotype
Rabbit Ig
Species Reactivity
Human
Concentration
0.25 mg/ml
Purification
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC
Suggested Dilutions
WB: 1:50~100; ELISA: 1: 1,000;
Background
RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.
Related Pathway
TGF Beta Signaling Pathway
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Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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