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Home Antibody All anti-RPS6KB1 antibodies

Anti-RPS6KB1 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA302197 Rabbit Polyclonal Antibody against RPS6KB1 (S424) 100ug $325 3-7 Days
LC401097 RPS6KB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
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Also for RPS6KB1 (NM_003161)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis S6K (RPS6KB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from human S6K (RPS6KB1).
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Mouse, Rat, Bovine, Rabbit) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:10~50
Predicted MW Explanation 59140 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1), transcript variant 1
Alternative Namep70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; S6K; S6K-beta-1; S6K1; STK14A
Database LinkNP_003152
Entrez Gene 6198 Human
Entrez Gene 72508 Mouse
Entrez Gene 83840 Rat
FunctionRPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.
Related PathwayProtein KinaseDruggable Genome ErbB signaling pathwaymTOR signaling pathwayTGF-beta signaling pathwayFc gamma R-mediated phagocytosisInsulin signaling pathwayAcute myeloid leukemiaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of RPS6KB1 (arrow) using rabbit polyclonal RPS6KB1 Antibody (S424) (RB11404). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2) (Origene Technologies).
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Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (TA302197) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 (arrow) was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (TA302197)
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) (Cat.#TA302197), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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