This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human PXN.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
Guaranteed Application *
WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
PXN is a cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. PXN contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).
EGFR1 Signaling Pathway
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(LEFT) Western blot analysis of PXN (arrow) using rabbit polyclonal PXN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PXN gene (Lane 2) (Origene Technologies). (RIGHT) Western blot analysis of PXN Antibody (Center) in Y79 cell line lysates (35ug/lane). PXN (arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PXN antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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