Home Antibody All anti-MAP3K5 antibodies
Also for MAP3K5 (NM_005923)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S83 of human MAP3K5.|
||WB: 1:50~100; IHC: 1: 10~50; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)|
|ASK1; MAPKKK5; MEKK5|
|Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.|
MAPK signaling pathway
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Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP3K5 Antibody (S83) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.