Home Antibody All anti-RAD9A antibodies
Also for RAD9A (NM_004584)
|This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Rad9.|
||WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens RAD9 homolog A (S. pombe) (RAD9A), transcript variant 1|
|This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.|
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(LEFT)Western blot analysis of anti-Rad9 BH3 domain Pab in HL-60 cell lysate. Rad9 BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. (RIGHT) Western blot analysis of Rad9(arrow) using rabbit polyclonal Rad9 BH3 domain Pab . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Rad9 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.