Home Antibody All anti-BNIP3L antibodies
Also for BNIP3L (NM_004331)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human NIP3.|
||WB: 1: 100~500; IHC: 1:50~100; IF: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens BCL2/adenovirus E1B 19kDa interacting protein 3-like (BNIP3L)|
|NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.|
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The anti-NIP3 BH3 domain Pab is used in Western blot to detect NIP3 BH3 in Ramos cell lysate (lane 1) and in mouse brain tissue lysate (lane 2).
TOP: Freshly isolated mouse hepatocytes plated on coverslips (2 x105 cells/22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with anti-BNIP3 antibody Cat # AP1321a. Data courtesy of Ruben Zamora, University of Pittsburgh. BOTTOM: Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.