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Home Antibody All anti-CD19 antibodies

Anti-CD19 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302072
  • Rabbit Polyclonal Antibody against CD19 (N-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400678) , 20ug Explanation
100ug 325 In Stock
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WB(3)
IHC(1)
IF(1)
FC(1)
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Also for CD19 (NM_001770)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis CD19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-172 amino acids from the N-terminal region of human CD19.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman Concentration0.25 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000, IF: 1:10~50, FC: 1:10~50, IHC: 1:10~50
Predicted MW Explanation 61128 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.

Reference Data

Target NameHomo sapiens CD19 molecule (CD19), transcript variant 2
Alternative NameB4; CVID3
Database LinkNP_001761
Entrez Gene 930 Human
FunctionLymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Related PathwayTransmembraneDruggable Genome Hematopoietic cell lineageB cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of CD19 (arrow) using rabbit polyclonal CD19 Antibody (N-term) (Cat.#TA302072). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD19 gene (Lane 2) (Origene Technologies).
WB Image
CD19 Antibody (N-term) (Cat.# TA302072) western blot analysis in Raji cell line lysates (35ug/lane).This demonstrates the CD19 antibody detected the CD19 protein (arrow).
WB Image
Western blot analysis of lysate from Ramos cell line, using CD19Antibody (N-term)(Cat. #TA302072). TA302072 was diluted at 1:500. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
IHC Image
Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Immunofluorescence analysis of CD19 Antibody (N-term) with paraffin-embedded human lymph tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
FC Image
Flow cytometric analysis of CEM cells using CD19 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

 

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